Closing the gap between in vivo and in vitro omics: using QA/QC to strengthen ex vivo NMR metabolomics. Amith Sadananda Maroli, Robert Powers.Engineering aspects of FlowNMR spectroscopy setups for online analysis of solution-phase processes. Thomlinson, Rachael Broomfield-Tagg, John P. Asad Saib, Alejandro Bara-EstaГєn, Owen J.Progress in Nuclear Magnetic Resonance Spectroscopy 2021, 126-127, 121-180. NMR spectroscopy of wastewater: A review, case study, and future potential. Helm, Sonya Kleywegt, Karl Jobst, Ronald Soong, Myrna J. Majumdar, Andersson Barison, Luciano Morais LiГЈo, Antonio Gilberto Ferreira, Venita Decker, Benjamin Goerling, Manfred Spraul, Markus Godejohann, Paul A. Lysak, Katelyn Downey, FlГЎvio Vinicius CrizГіstomo Kock, Xiang You, Rudraksha D. In Vivo Ultraslow MAS 2H/13C NMR Emphasizes Metabolites in Dynamic Flux. Simpson, Jochem Struppe, Hermann Heumann, Sebastian Schmidt, Holger Boenisch, Daniel Lane, Andre J.

Yalda Liaghati Mobarhan, Ronald Soong, Wolfgang Bermel, Myrna J.Selective Amino Acid-Only in Vivo NMR: A Powerful Tool To Follow Stress Processes. Daniel Lane, Ronald Soong, Wolfgang Bermel, Paris Ning, Rudraksha Dutta Majumdar, Maryam Tabatabaei-Anaraki, Hermann Heumann, Marcel Gundy, Holger BГ¶nisch, Yalda Liaghati Mobarhan, Myrna J.Understanding the Fate of Environmental Chemicals Inside Living Organisms: NMR-Based 13C Isotopic Suppression Selects Only the Molecule of Interest within 13C-Enriched Organisms. Daniel Lane, Yalda Liaghati Mobarhan, Ronald Soong, Paris Ning, Wolfgang Bermel, Maryam Tabatabaei Anaraki, Bing Wu, Hermann Heumann, Marcel Gundy, Holger Boenisch, Tae-Yong Jeong, Vera Kovacevic, Myrna J.Real-Time In-Organism NMR Metabolomics Reveals Different Roles of AMP-Activated Protein Kinase Catalytic Subunits. Chung, Sang-Min Jeon, Junho Lee, Sunghyouk Park. Tin Tin Manh Nguyen, Yong Jin An, Jin Wook Cha, Yoon-Joo Ko, Hanee Lee, Christine H.Targeting the Lowest Concentration of a Toxin That Induces a Detectable Metabolic Response in Living Organisms: Time-Resolved In Vivo 2D NMR during a Concentration Ramp. Daniel Lane, Wolfgang Bermel, Paris Ning, Tae-Yong Jeong, Richard Martin, Ronald Soong, Bing Wu, Maryam Tabatabaei-Anaraki, Hermann Heumann, Marcel Gundy, Holger Boenisch, Antonio Adamo, George Arhonditsis, AndrГ© J.5-Axis CNC Micromilling for Rapid, Cheap, and Background-Free NMR Microcoils. Vincent Moxley-Paquette, Daniel Lane, Ronald Soong, Paris Ning, Monica Bastawrous, Bing Wu, Maysam Zamani Pedram, Md Aminul Haque Talukder, Ebrahim Ghafar-Zadeh, Dimitri Zverev, Richard Martin, Bob Macpherson, Mike Vargas, Daniel Schmidig, Stephan Graf, Thomas Frei, Danijela Al Adwan-Stojilkovic, Peter De Castro, Falko Busse, Wolfgang Bermel, Till Kuehn, Rainer Kuemmerle, Michael Fey, Frank Decker, Henry Stronks, Ruby May A.This article is cited by 23 publications. The particular bloom used here was low in microcystins, and the metabolic stress impacts are consistent with the bloom being a poor food source forcing the Daphnia to utilize their own energy reserves. Clear metabolic changes are observed over a range of metabolites including carbohydrates, lipids, amino acids, and a nucleotide demonstrating in vivo NMR as a powerful tool to monitor environmental stress. To evaluate the feasibility and applicability of the flow system, changes in the metabolite profile of 13C enriched Daphnia magna over a 24-h period are compared when feeding laboratory food vs exposing them to a natural algal bloom sample. Using the flow system, the living organism can be kept alive without stress indefinitely. The use of a double pump design with a sump slurry pump return allows algal food suspensions to be continually supplied without the need for filters, eliminating the possibility of clogging and leaks. The system allows improved locking, shimming, and water suppression, as well as allowing the use of trace amounts of expensive toxic contaminants or low volumes of precious natural environmental samples as stressors. In this study, a novel continuous low-volume flow system, suitable for in vivo NMR metabolomics studies, is demonstrated. In vivo nuclear magnetic resonance (NMR) spectroscopy is a particularly powerful technique, since it allows samples to be analyzed in their natural, unaltered state, criteria paramount for living organisms.

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